Lipogro® Bovine Cholesterol Concentrate
Lipogro® Bovine Cholesterol Concentrate Solution is a growth enhancement supplement for cell and tissue culture over a range of media applications and monoclonal antibody production. Lipogro® can be used in mammalian cell culture as an FBS substitute or combined with cell culture media to reduce the amount of FBS or NBCS used. Lipogro® also works as a cell culture supplement on existing serum- reduced or serum-free media.
Derived from adult bovine serum lipid fractions, Lipogro® is a high-clarity, aqueous solution comprised of lipoproteins including purified cholesterol and essential fatty acids. RMBIO manufactures Lipogro® using a proprietary process of highly effective extraction and filtration steps to retain maximum product potency, two heating steps to ensure viral inactivation, and a final triple 0.1 µm filtration before dispensing the purified lipoprotein solution into sterile containers. The resulting product can increase cell growth, viability, and productivity.
- Essentially IgG free
- Cell culture supplement in Myeloma (NSO, Sp20), Hybridoma, HEK293, and MDCK cell lines
- Can supplement cell growth media or act as a FBS substitute
- Also used in production of veterinary vaccines, monoclonal antibodies, recombinant proteins, and live cellular therapies
- Solution is rich in lipoproteins, including purified cholesterol and essential fatty acids
- Manufactured at our GMP compliant and FDA registered facility
- Storage Conditions: 2 - 8° C
Lipogro® Bovine Cholesterol Concentrate specific product test requirements and typical values are given below:
Parameter | Method | Specification |
---|---|---|
Cholesterol Concentration | Chemistry Analyzer | 900 – 1100 mg/dL |
Total Protein | Chemistry Analyzer | ≤ 2.0 g/dL |
pH | pH Meter | 7.0 – 8.4 |
Endotoxin | Lumulus Amebocyte Lysate | ≤ 50 EU/ml |
Sterility | 9CFR 113.26 | No Growth |
Mycoplasma | 9CFR 113.28 | Not Detected |
Calcium | Chemistry Analyzer | ≤ 5.0 mg/dL |
IgG | ELISA | As Reported |
Viral Analysis | CFR 113.53 | Not Detected |
Appearance | Visual | Clear, Yellow-Amber |
Parameter | Method | Specification |
---|---|---|
Cholesterol Concentration | Chemistry Analyzer | 900 – 1100 mg/dL |
Total Protein | Chemistry Analyzer | ≤ 2.0 g/dL |
pH | pH Meter | 7.0 – 8.4 |
Endotoxin | Lumulus Amebocyte Lysate | ≤ 50 EU/ml |
Sterility | 9CFR 113.26 | No Growth |
Mycoplasma | 9CFR 113.28 | Not Detected |
Calcium | Chemistry Analyzer | ≤ 5.0 mg/dL |
IgG | ELISA | As Reported |
Viral Analysis | CFR 113.53 | Not Detected |
Appearance | Visual | Clear, Yellow-Amber |