DMEM 1X, High Glucose, With L-glutamine, With HEPES, With Sodium Pyruvate, With Pyridoxine HCI

DMEM 1X, High Glucose, With L-glutamine, With HEPES, With Sodium Pyruvate, With Pyridoxine HCI

DMEM (1X), liquid, high glucose, with L-glutamine, with pyridoxine hydrochloride, and with 110 mg/L sodium pyruvate.
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Catalog Number : DME-BBZ-XXX-002
Dulbecco’s Modified Eagle’s Medium (DMEM)
Size
Price
Quantity
500ml
$15.75
Case of 6, 500mL bottles
$80.32
 
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Product Details

Widely used for mammalian cell culture, Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional supplementary components, such as D-glucose. 

DME-BBZ-XXX-002 contains a high D-glucose concentration of 4500 mg/L, 584 mg/L L-glutamine, 4.0mg/L pyridoxine hydrochloride, and 110 mg/L sodium pyruvate. Important considerations when selecting a nutrient medium are: cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.

Storage/Stability

The liquid medium should be stored at 2~8°C

away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution

(2) Cloudy appearance

(3) Color change

(4) pH change

The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Precautions

For In Vitro Use Only


Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 5.9 - 6.4
Osmolality Chemistry Analyzer 320 - 355 mOsm/kg H2O
Endotoxin LAL ≤ 10 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
Glycine 30
L-Arginine Hydrochloride 84
L-Cystine 2HCl 63
L-Glutamine 584
L-Histidine Hydrochloride-H2O 42
L-Isoleucine 105
L-Leucine 105
L-Lysine Hydrochloride 146
L-Methionine 30
L-Phenylalanine 66
L-Serine 42
L-Threonine 95
L-Tryptophan 16
L-Tyrosine Disodium Salt Dihydrate 104
L-Valine 94
Choline Chloride 4
D-Calcium Pantothenate 4
Folic Acid 4
Niacinamide 4
Pyridoxine Hydrochloride 4
Riboflavin 0.4
Thiamine Hydrochloride 4
i-Inositol 7.2
Calcium Chloride (CaCl2) (anhyd.) 200
Ferric Nitrate (Fe(NO3)3”9H2O) 0.1
Magnesium Sulfate (MgSO4) (anhyd.) 97.67
Potassium Chloride (KCl) 400
Sodium Chloride (NaCl) 6400
Sodium Phosphate Monobasic (NaH2PO4-H2O) 125
D-Glucose (Dextrose) 4500
Phenol Red 15
Sodium Pyruvate 110