RMBIO uses premium grade FBS as the starting material for our IgG Depleted FBS. We then utilize a series of chromatography procedures to reduce the IgG content. The resulting FBS has an average IgG level of less than 5 µg/mL and cell growth performance comparable to the starting material. The average level of IgG in normal fetal calf serum is approximately 70-300 µg/mL.

• Gamma irradiated at 25-40kGy
• Total IgG content below 5 µg/mL
• IgG depleted FBS performs well in monoclonal antibody production
• Triple 0.1 µm filtered
• Large lot sizes >1000 Liters available
• Custom packaging sizes available upon request
• All fetal bovine serum is fully traceable and is sourced from audited suppliers
• Storage conditions: ≤ -10° C

Gamma Irradiation 101
Gamma irradiation is a physical inactivation approach used to achieve pathogen reduction in the serum. This mitigates risk from low-level contaminants that may not be detected through viral or mycoplasma testing. RMBIO serum is gamma irradiated via controlled exposure to Cobalt 60 at an industry standard dose of 25-40kGy. Most typical viral contaminants of bovine serum are readily inactivated at this irradiation range. Advantages of gamma irradiation include:

• No potential for residual contaminants
• Widely accepted by pharmaceutical and research industries
• Accepted by regulatory agencies
• Minimal effect on product performance
• Effective virus inactivation

IgG Depleted Fetal Bovine Serum (FBS) specific product test requirements and typical values are given below: