MEM-003: MEM 1X with Earle's Salts, with L-Glutamine, with nonessential amino acids, with HEPES
Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for a wide variety of cells grown in monolayer. Modified from Basal Medium Eagle (BME) to cultivate fastidious cells like normal mammalian fibroblasts and certain subtypes of HeLa cells, MEM includes higher concentrations of amino acids to achieve a protein composition similar to cultured mammalian cells. MEM containing either Hanks’ or Earle’s salts can be supplemented with non-essential amino acids for use with a variety of cell types. The formulation can be further modified by eliminating calcium, necessary for attachment, to permit growth of cells in suspension culture.
MEM-BBZ-XXX-003 is based on Earle’s balanced salts and contains 0.1 mM each (1X) of non-essential amino acids, 100X, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycine, L-proline, and L-serine) and 292 mg/L L-glutamine. MEM-BBZ-XXX-003 is used to culture cells that are unable to manufacture or retain non-essential amino acids. Important considerations when selecting a nutrient medium are: cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.
Storage/Stability
The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:
(1) Precipitate or particulate matter throughout the solution
(2) Cloudy appearance
(3) Color change
(4) pH change
The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Precautions
For research use only.| Parameter | Method | Specification |
|---|---|---|
| Appearance | Visual | Red Transparent Solution |
| pH at RT Before Additions | pH Meter | 7.0 - 7.6 |
| Osmolality | Chemistry Analyzer | 279-329 mOsm/kg H2O |
| Endotoxin | LAL | ≤ 10 EU/ml |
| Sterility | Sterilized by 0.2 um filtration system |
| CaCl2 (anhydrous) | 200.00 |
| KCl | 400.00 |
| MgSO4 (anhydrous) | 97.67 |
| NaCI | 6800.00 |
| NaHCO3 | 2200.00 |
| NaH2PO4·H2O | 140.00 |
| D-Glucose | 1000.00 |
| HEPES | 5958.00 |
| Phenol Red | 10.00 |
| L-Alanine* | 8.90 |
| L-Arginine·HCl* | 126.00 |
| L-Asparagine·H2O* | 15.00 |
| L-Aspartic Acid* | 13.30 |
| L-Cystine·2HCl* | 31.28 |
| Glycine* | 7.50 |
| L-Glutamic Acid* | 14.70 |
| L-Glutamine* | 292.00 |
| L-Histidine·HCl·H2O | 42.00 |
| L-Isoleucine | 52.00 |
| L-Leucine | 52.00 |
| L-Lysine·HCl | 72.50 |
| L-Methionine | 15.00 |
| L-Phenylalanine | 32.00 |
| L-Proline* | 11.50 |
| L-Serine* | 10.50 |
| L-Threonine | 48.00 |
| L-Tryptophan | 10.00 |
| L-Tyrosine·2Na·2H2O* | 51.90 |
| L-Valine | 46.00 |
| D-Ca Pantothenate | 1.00 |
| Choline Chloride | 1.00 |
| Folic Acid | 1.00 |
| i-Inositol | 2.00 |
| Niacinamide | 1.00 |
| Pyridoxal·HCl | 1.00 |
| Riboflavin | 0.10 |
| Thiamine·HCl | 1.00 |