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MEM-002: MEM Alpha Modification With L-glutamine
MEM-002: MEM Alpha Modification With L-glutamine

MEM-002: MEM Alpha Modification With L-glutamine

Minimum Essential Medium Eagle, Alpha Modification (Alpha MEM) (1X), with L-glutamine, and without ribonucleosides and deoxyribonucleosides.
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Catalog Number : MEM-BBZ-XXX-002
Alpha MEM With L-glutamine
Size
Price
Quantity
500ml
$40.00
Case of 6 500ml bottles
$204.00
1L
$64.00
 
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Product Details

Minimum Essential Medium, Alpha Modification (Alpha MEM) contains additional amino acids and vitamins required for transfection of foreign DNA to mammalian cells. Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for a wide variety of cells grown in monolayer. Modified from Basal Medium Eagle (BME) to cultivate fastidious cells like normal mammalian fibroblasts and certain subtypes of HeLa cells, MEM includes higher concentrations of amino acids to achieve a protein composition similar to cultured mammalian cells. MEM containing either Hanks’ or Earle’s salts can be supplemented with non-essential amino acids for use with a variety of cell types. The formulation can be further modified by eliminating calcium, necessary for attachment, to permit growth of cells in suspension culture.

MEM-BBZ-XXX-002 is based on Earle’s balanced salts and contains 292 mg/L of L-glutamine, but no deoxyribonucleosides and no ribonucleosides.

Storage/Stability

The liquid medium should be stored at 2-8°C away from direct sunlight. Deterioration of the liquid medium may be recognized by:

  • Precipitate or particulate matter visible throughout the solution
  • Cloudy appearance
  • Color change
  • pH change

The nature of supplements added may affect the storage conditions and shelf life of the medium. Product label bears expiration date.

Precautions

For In Vitro Use Only


Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 7.0 - 7.6
Osmolality Chemistry Analyzer 284-314 mOsm/kg H2O
Endotoxin LAL ≤ 10 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 200.00
KCl 400.00
MgSO4 (anhydrous) 97.67
NaCI 6800.00
NaHCO3 2200.00
NaH2PO4 (anhydrous) 140.00
D-Glucose 1000.00
Lipoic acid 0.20
Phenol Red 10.00
Sodium Pyruvate 110.00
L-Alanine 25.00
L-Arginine·HCl 127.00
L-Asparagine·H2O 50.00
L-Aspartic Acid 30.00
L-Cystine·2HCl 24.00
L-Cystine·HCl·H2O 100.00
Glycine 50.00
L-Glutamic Acid 75.00
L-Glutamine 292.00
L-Histidine·HCl·H2O 42.00
L-Isoleucine 52.40
L-Leucine 52.40
L-Lysine·HCl 72.00
L-Methionine 15.00
L-Phenylalanine 32.00
L-Proline 40.00
L-Serine 25.00
L-Threonine 48.00
L-Tryptophan 10.00
L-Tyrosine·2Na·2H2O 52.00
L-Valine 46.00
L-Ascorbic Acid 50.00
Biotin 0.10
Choline Chloride 1.00
Folic Acid 1.00
i-Inositol 2.00
Niacinamide 1.00
D-Ca Pantothenate 1.00
Pyridoxal·HCl 1.00
Riboflavin 0.10
Thiamine·HCl 1.00
Vitamin B12 1.36
Adenosine 0.00
Cytidine 0.00
Guanosine 0.00
Uridine 0.00
2’-Deoxyadenosine 0.00
2’-Deoxycytidine·HCl 0.00
2’-Deoxyguanosine 0.00
Thymidine 0.00

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