HMF-000: Ham’s F-12, 1X Kaighn’s Modification
A modified version of Ham’s F-12 medium, Ham’s F-12K (Kaighn’s) Medium was originally formulated for use with or without supplemental serum. Adding components like putrescine, thymidine, hypoxanthine, zinc, and higher levels of sodium pyruvate and amino acids than found in traditional media, can compensate for the lack of serum. Depending on the cell type cultured however, FBS and growth factors are often added to the medium.
HMF-BBZ-XXX-000 contains 292 mg/L L-glutamine and 2500 mg/L sodium bicarbonate.
Storage/Stability
The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:
(1) Precipitate or particulate matter throughout the solution
(2) Cloudy appearance
(3) Color change
(4) pH change
The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Precautions
For research use only.
| Parameter | Method | Specification |
|---|---|---|
| Appearance | Visual | Red Transparent Solution |
| pH at RT Before Additions | pH Meter | 7.0 - 7.6 |
| Osmolality | Chemistry Analyzer | 320 - 355 mOsm/kg H2O |
| Endotoxin | LAL | ≤ 1 EU/ml |
| Sterility | Sterilized by 0.2 um filtration system |
| CaCl2 (anhydrous) | 135.24 |
| KH2PO4 | 58.523 |
| CuSO4·5H2O | 0.0025 |
| FeSO4·7H2O | 0.834 |
| KCl | 283.29 |
| MgCl2·6H2O | 105.716 |
| MgSO4 | 192.64 |
| NaCl | 7597.2 |
| NaHCO3 | 2500.00 |
| Na2HPO4 (anhydrous) | 115.02 |
| ZnSO4·7H2O | 0.14375 |
| D-Glucose | 1260.00 |
| HEPES | 0.00 |
| Hypoxanthine·Na | 4.083 |
| Linoleic Acid | 0.00 |
| DL-6,8-Thioctic Acid | 0.02063 |
| Phenol Red | 3.31806 |
| Putrescine·HCl | 0.3222 |
| Sodium Pyruvate | 220.00 |
| Thymidine | 0.7266 |
| L-Alanine | 17.818 |
| L-Arginine·HCl | 421.40 |
| L-Asparagine·H2O | 30.02 |
| L-Aspartic Acid | 26.62 |
| L-Cysteine·HCl·H2O | 70.24 |
| L-Glutamic Acid | 29.42 |
| L-Glutamine | 292.20 |
| Glycine | 15.014 |
| L-Histidine·3HCl·H2O | 41.92 |
| L-Isoleucine | 7.872 |
| L-Leucine | 26.24 |
| L-Lysine·HCl | 73.04 |
| L-Methionine | 8.952 |
| L-Phenylalanine | 9.912 |
| L-Proline | 69.06 |
| L-Serine | 21.02 |
| L-Threonine | 23.82 |
| L-Tryptophan | 4.084 |
| L-Tyrosine·2Na·2H2O | 10.872 |
| L-Valine | 23.42 |
| Biotin | 0.07329 |
| Calcium D-pantothenate | 0.477 |
| Choline Chloride | 13.96 |
| Folic Acid | 1.3242 |
| Myo-Inositol | 18.02 |
| Niacinamide | 0.03663 |
| Pyridoxine HCI | 0.06168 |
| Riboflavin | 0.03764 |
| Thiamine HCI | 0.3373 |
| Vitamin B12 | 13.96 |