DMEM-005: DMEM/F-12 Ham 1:1 Mixture 1X, With L-glutamine, Without HEPES
F-12 Nutrient Mixture (Ham’s F-12) is a serum-free medium that relies on a combination of nutrient supplements, growth factors, and hormones to culture cells. During the past decade, researchers have reported successful culture of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin, epidermal growth factor (EGF), leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. A 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and Ham’s F-12 Nutrient Mixture was found to be most satisfactory for studies of this type.
DME-BBZ-XXX-005 contains 365 mg/L L-glutamine but no HEPES.
Storage/Stability
The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:
(1) Precipitate or particulate matter throughout the solution
(2) Cloudy appearance
(3) Color change
(4) pH change
The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Precautions
For research use only.
| Parameter | Method | Specification |
|---|---|---|
| Appearance | Visual | Red Transparent Solution |
| pH at RT Before Additions | pH Meter | 6.9 - 7.5 |
| Osmolality | Chemistry Analyzer | 268 - 312 mOsm/kg H2O |
| Endotoxin | LAL | ≤ 1 EU/ml |
| Sterility | Sterilized by 0.2 um filtration system |
| CaCl2 (anhydrous) | 116.61 |
| CuSO4·5H2O | 0.0013 |
| Fe(NO3)3·9H2O | 0.05 |
| FeSO4·7H2O | 0.417 |
| KCl | 311.80 |
| MgCl2 | 28.61 |
| MgSO4 (anhydrous) | 48.84 |
| NaCl | 6999.50 |
| NaHCO3 | 1200.00 |
| NaH2PO4·H2O | 62.50 |
| Na2HPO4 (anhydrous) | 71.02 |
| ZnSO4·7H2O | 0.43 |
| D-Glucose | 3151.00 |
| HEPES | 0.00 |
| Na·Hypoxanthine | 2.70 |
| Linoleic Acid | 0.04 |
| DL-α-Lipoic Acid | 0.105 |
| Phenol Red-Na | 8.60 |
| Sodium Putrescine·2HCl | 0.08 |
| Sodium Pyruvate | 55.00 |
| Thymidine | 0.37 |
| L-Alanine | 4.45 |
| L-Arginine·HCl | 147.50 |
| L-Asparagine·H2O | 7.501 |
| L-Aspartic Acid | 6.65 |
| L-Cysteine·HCl·H2O | 17.56 |
| L-Cystine·2HCl | 31.29 |
| Glycine | 18.75 |
| L-Glutamic Acid | 7.35 |
| L-Glutamine | 365.00 |
| L-Histidine·HCl·H2O | 31.48 |
| L-Isoleucine | 54.47 |
| L-Leucine | 59.05 |
| L-Lysine·HCl | 91.26 |
| L-Methionine | 17.24 |
| L-Phenylalanine | 35.48 |
| L-Proline | 17.25 |
| L-Serine | 26.25 |
| L-Threonine | 53.45 |
| L-Tryptophan | 9.02 |
| L-Tyrosine·2Na·2H2O | 55.79 |
| L-Valine | 52.85 |
| Biotin | 0.0036 |
| Calcium D-pantothenate | 2.24 |
| Folic Acid | 2.65 |
| Myo-inositol | 12.60 |
| Niacinamide | 2.02 |
| Pyridoxine-HCl | 2.03 |
| Riboflavin | 0.22 |
| Thiamine-HCI | 2.17 |
| Vitamin B12 | 0.68 |