Ham’s F-12, 1X Kaighn’s Modification

Ham’s F-12, 1X Kaighn’s Modification

F-12 Nutrient Mixtures Ham (Ham’s F-12) 1X Kaighn’s Modification with L-glutamine
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Catalog Number : HMF-BBZ-XXX-000
Ham’s F-12, 1X Kaighn’s Modification
Size
Price
Quantity
500ml
$17.50
Case of 6 500ml bottles
$89.25
1L
$28.00
 
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Product Details

A modified version of Ham’s F-12 medium, Ham’s F-12K (Kaighn’s) Medium was originally formulated for use with or without supplemental serum. Adding components like putrescine, thymidine, hypoxanthine, zinc, and higher levels of sodium pyruvate and amino acids than found in traditional media, can compensate for the lack of serum. Depending on the cell type cultured however, FBS and growth factors are often added to the medium.

HMF-BBZ-XXX-000 contains 292 mg/L L-glutamine and 2500 mg/L sodium bicarbonate.

Storage/Stability

The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution

(2) Cloudy appearance

(3) Color change

(4) pH change

The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Precautions

For In Vitro Use Only


Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 7.0 - 7.6
Osmolality Chemistry Analyzer 320 - 355 mOsm/kg H2O
Endotoxin LAL ≤ 1 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 135.24
KH2PO4 58.523
CuSO4·5H2O 0.0025
FeSO4·7H2O 0.834
KCl 283.29
MgCl2·6H2O 105.716
MgSO4 192.64
NaCl 7597.2
NaHCO3 2500.00
Na2HPO4 (anhydrous) 115.02
ZnSO4·7H2O 0.14375
D-Glucose 1260.00
HEPES 0.00
Hypoxanthine·Na 4.083
Linoleic Acid 0.00
DL-6,8-Thioctic Acid 0.02063
Phenol Red 3.31806
Putrescine·HCl 0.3222
Sodium Pyruvate 220.00
Thymidine 0.7266
L-Alanine 17.818
L-Arginine·HCl 421.40
L-Asparagine·H2O 30.02
L-Aspartic Acid 26.62
L-Cysteine·HCl·H2O 70.24
L-Glutamic Acid 29.42
L-Glutamine 292.20
Glycine 15.014
L-Histidine·3HCl·H2O 41.92
L-Isoleucine 7.872
L-Leucine 26.24
L-Lysine·HCl 73.04
L-Methionine 8.952
L-Phenylalanine 9.912
L-Proline 69.06
L-Serine 21.02
L-Threonine 23.82
L-Tryptophan 4.084
L-Tyrosine·2Na·2H2O 10.872
L-Valine 23.42
Biotin 0.07329
Calcium D-pantothenate 0.477
Choline Chloride 13.96
Folic Acid 1.3242
Myo-Inositol 18.02
Niacinamide 0.03663
Pyridoxine HCI 0.06168
Riboflavin 0.03764
Thiamine HCI 0.3373
Vitamin B12 13.96