Leibovitz L-15 Medium

Leibovitz L-15 Medium

Leibovitz L-15 medium with 300 mg/L L-glutamine.
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Catalog Number : LBZ-BBZ-XXX-000
Leibovitz L-15 Medium
Size
Price
Quantity
500ml
$26.00
Case of 6 500ml bottles
$132.50
1L
$42.00
 
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Product Details

Leibovitz L-15 medium was originally developed in 1963 for use in a carbon dioxide (CO2) free atmosphere. Specialty incubators which buffered most media in a CO2 enriched environment were not readily available in at the time. This lead Dr. Leibovitz to develop a buffering system based on salts, free base amino acids, and galactose instead of glucose to help maintain pH levels. L-15 medium can support many established cell lines and cultivate various viruses.

LBZ-BBZ-XXX-000 contains 300 mg/L L-glutamine. Important considerations when selecting a nutrient medium include: cell type, culture type (i.e., monolayer, suspension, or clonal) and the degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium type, required supplementation and physiological parameters required for a specific cell line.

 

Storage/Stability

The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution

(2) Cloudy appearance

(3) Color change

(4) pH change

The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

 

Precautions

For In Vitro Use Only

Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 7.3 - 7.9
Osmolality Chemistry Analyzer 304 - 336 mOsm/kg H2O
Endotoxin LAL ≤ 1 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 140.00
KCl 400.00
KH2PO4 60.00
MgCl2 (anhydrous) 93.68
MgSO4 (anhydrous) 97.67
NaCl 8000.00
Na2HPO4 190.00
D-(+)-Galactose 900.00
Phenol Red-Na 10.65
Sodium Pyruvate 550.00
L-Alanine 225.00
L-Arginine (free base) 500.00
L-Asparagine (anhydrous) 250.00
L-Cysteine (free base) 120.00
Glycine 200.00
L-Glutamine 300.00
L-Histidine (free base) 250.00
L-Isoleucine 125.00
L-Leucine 125.00
L-Lysine (free base) 75.00
L-Methionine 75.00
L-Phenylalanine 125.00
L-Serine 200.00
L-Threonine 300.00
L-Tryptophan 20.00
L-Tyrosine (free base) 300.00
L-Valine 100.00
D-Ca Pantothenate 1.00
Choline Chloride 1.00
Folic Acid 1.00
Myo-Inositol 2.00
Niacinamide 1.00
Pyridoxine·HCl 1.00
Riboflavin-5PO4-Na 0.10
Thiamine PO4-Cl-2H2O 1.00