MEM 1X with Earle's Salts, with L-Glutamine, with nonessential amino acids, with HEPES

MEM 1X with Earle's Salts, with L-Glutamine, with nonessential amino acids, with HEPES

Based on Earle’s balanced salts with 0.1 mM each (1X) of non-essential amino acids, 100X, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycine, L-proline, and L-serine) and 292 mg/L L-glutamine.
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500ml
$24.00
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Case of 6 500ml bottles
$122.00
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1L
$38.00
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Product Details

Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for a wide variety of cells grown in monolayer. Modified from Basal Medium Eagle (BME) to cultivate fastidious cells like normal mammalian fibroblasts and certain subtypes of HeLa cells, MEM includes higher concentrations of amino acids to achieve a protein composition similar to cultured mammalian cells. MEM containing either Hanks’ or Earle’s salts can be supplemented with non-essential amino acids for use with a variety of cell types. The formulation can be further modified by eliminating calcium, necessary for attachment, to permit growth of cells in suspension culture.

MEM-BBZ-XXX-003 is based on Earle’s balanced salts and contains 0.1 mM each (1X) of non-essential amino acids, 100X, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycine, L-proline, and L-serine) and 292 mg/L L-glutamine. MEM-BBZ-XXX-003 is used to culture cells that are unable to manufacture or retain non-essential amino acids. Important considerations when selecting a nutrient medium are: cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.

Storage/Stability

The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution

(2) Cloudy appearance

(3) Color change

(4) pH change

The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Precautions

For In Vitro Use Only

Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 7.0 - 7.6
Osmolality Chemistry Analyzer 279-329 mOsm/kg H2O
Endotoxin LAL ≤ 10 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 200.00
KCl 400.00
MgSO4 (anhydrous) 97.67
NaCI 6800.00
NaHCO3 2200.00
NaH2PO4·H2O 140.00
D-Glucose 1000.00
HEPES 5958.00
Phenol Red 10.00
L-Alanine* 8.90
L-Arginine·HCl* 126.00
L-Asparagine·H2O* 15.00
L-Aspartic Acid* 13.30
L-Cystine·2HCl* 31.28
Glycine* 7.50
L-Glutamic Acid* 14.70
L-Glutamine* 292.00
L-Histidine·HCl·H2O 42.00
L-Isoleucine 52.00
L-Leucine 52.00
L-Lysine·HCl 72.50
L-Methionine 15.00
L-Phenylalanine 32.00
L-Proline* 11.50
L-Serine* 10.50
L-Threonine 48.00
L-Tryptophan 10.00
L-Tyrosine·2Na·2H2O* 51.90
L-Valine 46.00
D-Ca Pantothenate 1.00
Choline Chloride 1.00
Folic Acid 1.00
i-Inositol 2.00
Niacinamide 1.00
Pyridoxal·HCl 1.00
Riboflavin 0.10
Thiamine·HCl 1.00