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MEM 1X with Earle's Balanced Salts, without L-glutamine and without HEPES in a transparent 500 milliliter bottle.
MEM 1X with Earle's Balanced Salts, without L-glutamine and without HEPES in a transparent 1000 milliliter bottle.

MEM-004: MEM 1X with Earle's Salts, without L-glutamine, without HEPES

Based on Earle’s balanced salts without L-glutamine or HEPES.
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Case of 6 500ml bottles
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Product Details

Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for a wide variety of cells grown in monolayer. Modified from Basal Medium Eagle (BME) to cultivate fastidious cells like normal mammalian fibroblasts and certain subtypes of HeLa cells, MEM includes higher concentrations of amino acids to achieve a protein composition similar to cultured mammalian cells. MEM containing either Hanks’ or Earle’s salts can be supplemented with non-essential amino acids for use with a variety of cell types. The formulation can be further modified by eliminating calcium, necessary for attachment, to permit growth of cells in suspension culture.

MEM-BBZ-XXX-004 is based on Earle’s balanced salts and does not contain L-glutamine or HEPES. Important considerations when selecting a nutrient medium are: cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.


The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution

(2) Cloudy appearance

(3) Color change

(4) pH change

The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.


For research use only.

Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 7.0 - 7.6
Osmolality Chemistry Analyzer 276 - 306 mOsm/kg H2O
Endotoxin LAL ≤ 1.0 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 200.00
KCl 400.00
MgSO4 (anhydrous) 97.67
NaCI 6800.00
NaHCO3 2200.00
KH2PO4 60.00
NaH2PO4H2O 140.00
D-Glucose 1000.00
HEPES 0.00
Phenol Red 11.00
L-Alanine 0.00
L-Arginine·HCl 126.98
L-Asparagine·H2O 0.00
L-Aspartic Acid 0.00
L-Cystine-HCl-H20 0.00
L-Cystine-2HCl 31.28
Glycine 0.00
L-Glutamic Acid 0.00
L-Glutamine 0.00
L-Histidine·HCl·H2O 41.88
L-Isoleucine 52.00
L-Leucine 52.00
L-Lysine·HCl 72.47
L-Methionine 15.00
L-Phenylalanine 32.00
L-Proline 0.00
L-Serine 0.00
L-Threonine 48.00
L-Tryptophan 10.00
L-Tyrosine·2Na·2H2O 51.90
L-Valine 46.00
D-Ca Pantothenate 1.00
Choline Chloride 1.00
Folic Acid 1.00
Myo-inositol 2.00
Niacinamide 1.00
Pyridoxal·HCl 1.00
Riboflavin 0.10
Thymidine 1.00

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