MEM-002: MEM Alpha Modification With L-glutamine
Minimum Essential Medium, Alpha Modification (Alpha MEM) contains additional amino acids and vitamins required for transfection of foreign DNA to mammalian cells. Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for a wide variety of cells grown in monolayer. Modified from Basal Medium Eagle (BME) to cultivate fastidious cells like normal mammalian fibroblasts and certain subtypes of HeLa cells, MEM includes higher concentrations of amino acids to achieve a protein composition similar to cultured mammalian cells. MEM containing either Hanks’ or Earle’s salts can be supplemented with non-essential amino acids for use with a variety of cell types. The formulation can be further modified by eliminating calcium, necessary for attachment, to permit growth of cells in suspension culture.
MEM-BBZ-XXX-002 is based on Earle’s balanced salts and contains 292 mg/L of L-glutamine, but no deoxyribonucleosides and no ribonucleosides.
Storage/Stability
The liquid medium should be stored at 2-8°C away from direct sunlight. Deterioration of the liquid medium may be recognized by:
- Precipitate or particulate matter visible throughout the solution
- Cloudy appearance
- Color change
- pH change
The nature of supplements added may affect the storage conditions and shelf life of the medium. Product label bears expiration date.
Precautions
For In Vitro Use Only
| Parameter | Method | Specification |
|---|---|---|
| Appearance | Visual | Red Transparent Solution |
| pH at RT Before Additions | pH Meter | 7.0 - 7.6 |
| Osmolality | Chemistry Analyzer | 284-314 mOsm/kg H2O |
| Endotoxin | LAL | ≤ 10 EU/ml |
| Sterility | Sterilized by 0.2 um filtration system |
| CaCl2 (anhydrous) | 200.00 |
| KCl | 400.00 |
| MgSO4 (anhydrous) | 97.67 |
| NaCI | 6800.00 |
| NaHCO3 | 2200.00 |
| NaH2PO4 (anhydrous) | 140.00 |
| D-Glucose | 1000.00 |
| Lipoic acid | 0.20 |
| Phenol Red | 10.00 |
| Sodium Pyruvate | 110.00 |
| L-Alanine | 25.00 |
| L-Arginine·HCl | 127.00 |
| L-Asparagine·H2O | 50.00 |
| L-Aspartic Acid | 30.00 |
| L-Cystine·2HCl | 24.00 |
| L-Cystine·HCl·H2O | 100.00 |
| Glycine | 50.00 |
| L-Glutamic Acid | 75.00 |
| L-Glutamine | 292.00 |
| L-Histidine·HCl·H2O | 42.00 |
| L-Isoleucine | 52.40 |
| L-Leucine | 52.40 |
| L-Lysine·HCl | 72.00 |
| L-Methionine | 15.00 |
| L-Phenylalanine | 32.00 |
| L-Proline | 40.00 |
| L-Serine | 25.00 |
| L-Threonine | 48.00 |
| L-Tryptophan | 10.00 |
| L-Tyrosine·2Na·2H2O | 52.00 |
| L-Valine | 46.00 |
| L-Ascorbic Acid | 50.00 |
| Biotin | 0.10 |
| Choline Chloride | 1.00 |
| Folic Acid | 1.00 |
| i-Inositol | 2.00 |
| Niacinamide | 1.00 |
| D-Ca Pantothenate | 1.00 |
| Pyridoxal·HCl | 1.00 |
| Riboflavin | 0.10 |
| Thiamine·HCl | 1.00 |
| Vitamin B12 | 1.36 |
| Adenosine | 0.00 |
| Cytidine | 0.00 |
| Guanosine | 0.00 |
| Uridine | 0.00 |
| 2’-Deoxyadenosine | 0.00 |
| 2’-Deoxycytidine·HCl | 0.00 |
| 2’-Deoxyguanosine | 0.00 |
| Thymidine | 0.00 |