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DMEM/F-12 Ham 1:1 Mixture 1X with L-glutamine, with HEPES, without Sodium Bicarbonate in a 500 milliliter bottle.
DMEM/F-12 Ham 1:1 Mixture 1X with L-glutamine, with HEPES, without Sodium Bicarbonate in a 1000 milliliter bottle.

DMEM-009: DMEM/F-12 Ham 1:1 Mixture 1X, With L-glutamine, With HEPES, Without Sodium Bicarbonate

Dulbecco’s Modified Eagle’s Medium/F-12 Nutrient Mixture Ham (DMEM/F-12) 1:1 Mixtures, With 15mM HEPES, With 2.5mM L-glutamine, Without Sodium Bicarbonate
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Catalog Number : DME-BBZ-XXX-009
DMEM F-12 Ham 1:1 Nutrient Mixture 1X, With L-glutamine, With HEPES, Without Sodium Bicarbonate
Case of 6 500ml bottles
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Product Details

F-12 Nutrient Mixture (Ham’s F-12) is a serum-free medium that relies on a combination of nutrient supplements, growth factors, and hormones to culture cells. During the past decade, researchers have reported successful culture of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin, epidermal growth factor (EGF), leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. A 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and Ham’s F-12 Nutrient Mixture was found to be most satisfactory for studies of this type.

DME-BBZ-XXX-009 contains 365 mg/L L-glutamine and 15mM HEPES to compensate for the loss of buffering capacity incurred by eliminating serum. DME-BBZ-XXX-009 does not contain sodium bicarbonate.


The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution

(2) Cloudy appearance

(3) Color change

(4) pH change

The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.


For research use only.

Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 7.0 - 7.6
Osmolality Chemistry Analyzer 267 - 297 mOsm/kg H2O
Endotoxin LAL ≤ 1 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 116.61
CuSO4·5H2O 0.0013
Fe(NO3)3·9H2O 0.05
FeSO4·7H2O 0.417
KCl 311.80
MgCl2 28.61
MgSO4 (anhydrous) 48.84
NaCl 6999.50
NaHCO3 0.00
NaH2PO4·H2O 62.50
Na2HPO4 (anhydrous) 71.02
ZnSO4·7H2O 0.43
D-Glucose 3151.00
HEPES 3575.00
Hypoxanthine-2Na 2.70
Linoleic Acid 0.04
DL-α-Lipoic Acid 0.105
Phenol Red-Na 8.60
Putrescine·2HCl 0.08
Sodium Pyruvate 55.00
Thymidine 0.37
L-Alanine 4.45
L-Arginine·HCl 147.50
L-Asparagine·H2O 7.501
L-Aspartic Acid 6.65
L-Cysteine·HCl·H2O 17.56
L-Cystine·2HCl 31.29
Glycine 18.75
L-Glutamic Acid 7.35
L-Glutamine 365.00
L-Histidine·HCl·H2O 31.48
L-Isoleucine 54.47
L-Leucine 59.05
L-Lysine·HCl 91.26
L-Methionine 17.24
L-Phenylalanine 35.48
L-Proline 17.25
L-Serine 26.25
L-Threonine 53.45
L-Tryptophan 9.02
L-Tyrosine·2Na·2H2O 55.79
L-Valine 52.85
D-Biotin 0.0036
Calcium D-pantothenate 2.24
Choline Chloride 8.98
Folic Acid 2.65
Myo-Inositol 12.60
Niacinamide 2.02
Pyridoxine·HCI 2.03
Riboflavin 0.22
Thiamine·HCI 2.17
Vitamin B12 0.68

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