Minimum Essential Medium Eagle (MEM) (1X), liquid

Minimum Essential Medium Eagle (MEM) (1X), liquid

MEM with 0.1 mM each (1X) of non-essential amino acids, 100X, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycine, L-proline, and L-serine) and 292 mg/L L-glutamine.
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Catalog Number : MEM-BBZ-XXX-000
Minimum Essential Medium (MEM)
Case of 6, 500mL bottles
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Product Details

Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for a wide variety of cells grown in monolayer. Modified from Basal Medium Eagle (BME) to cultivate fastidious cells like normal mammalian fibroblasts and certain subtypes of HeLa cells, MEM includes higher concentrations of amino acids to achieve a protein composition similar to cultured mammalian cells. MEM containing either Hanks’ or Earle’s salts can be supplemented with non-essential amino acids for use with a variety of cell types. The formulation can be further modified by eliminating calcium, necessary for attachment, to permit growth of cells in suspension culture.

MEM-BBZ-XXX-000 is based on Earle’s balanced salts and contains 0.1 mM each (1X) of non-essential amino acids, 100X, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycine, L-proline, L-serine, and 292 mg/L L-glutamine.

MEM-BBZ-XXX-000 is used to culture cells that are unable to manufacture or retain non-essential amino acids. Important considerations when selecting a nutrient medium are: cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.

Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 5.05 ± 0.25
Osmolality Chemistry Analyzer 270-325 mOsm/kg H2O
Endotoxin LAL ≤ 10 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 200
KCl 400
MgSO4 (anhydrous) 97.67
NaCI 6800
NaHCO3 2200
NaH2PO4·H2O 140
D-Glucose 1000
Phenol Red 10
L-Alanine 8.9
L-Arginine·HCl 126
L-Asparagine·H2O 15
L-Aspartic Acid 13.3
L-Cystine·2HCl 31.28
Glycine 7.5
L-Glutamic Acid 14.7
L-Glutamine 292
L-Histidine·HCl·H2O 42
L-Isoleucine 52
L-Leucine 52
L-Lysine·HCl 72.5
L-Methionine 15
L-Phenylalanine 32
L-Proline 11.5
L-Serine 10.5
L-Threonine 48
L-Tryptophan 10
L-Tyrosine·2Na·2H2O 51.9
L-Valine 46
Choline Chloride 1
D-Ca Pantothenate 1
Folic Acid 1
i-Inositol 2
Niacinamide 1
Pyridoxal·HCl 1
Riboflavin 0.10
Thiamine·HCl 1