RPMI 1640 Media

RPMI 1640 Media

RPMI 1640 Medium (1X) liquid, with L-glutamine, without HEPES.
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Catalog Number : RPM-BBZ-XXX-000
RPMI 1640 Medium
Size
Price
Quantity
500ml
$16.00
Case of 6, 500mL bottles
$72.60
 
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Product Details

RPMI 1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, (hence the acronym RPMI) for long-term cell culture. The formulation is based on the RPMI 1630 series of media utilizing a bicarbonate buffering system with alterations to the amounts of amino acids and vitamins. RPMI 1640 medium is a modification of McCoy’s 5A medium and supports growth of many cell types, especially suspension cells such as normal and neoplastic leukocytes.

RPM-BBZ-5XM-000 contains 300 mg/L L-glutamine. Important considerations when selecting a nutrient medium are cell type,  culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Store at 2-8°C.


Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 7.5 - 8.5
Osmolality Chemistry Analyzer 257 ~ 299 mOsm/kg H2O
Endotoxin LAL ≤ 10 EU/mL
Sterility Sterilized by 0.2 um filtration system

Components mg/L
Ca(NO3)2·4H2O 100
KCl 400
MgSO4 (anhydrous) 48.84
NaCl 6000
NaHCO3 2000
NaH2PO4 (anhydrous) 800
D-Glucose 2000
Glutathione (reduced) 1
HEPES -
Phenol Red 5
L-Arginine 200
L-Asparagine (free base) 50
L-Aspartic Acid 20
L-Cystine·2HCl 65
L-Glutamic Acid 20
L-Glutamine 300
Glycine 10
L-Histidine (free base) 15
L-Hydroxyproline 20
L-Isoleucine 50
L-Leucine 50
L-Lysine·HCl 40
L-Methionine 15
L-Phenylalanine 15
L-Proline 20
L-Serine 30
L-Threonine 20
L-Tryptophan 5
L-Tyrosine·2Na·2H2O 29
L-Valine 20
Biotin 0.2
D-Ca Panto thenate 0.25
Choline Chloride 3
Folic Acid 1
i-Inositol 35
Niacinamide 1
Para-aminobenzoic Acid 1
Pyridoxine·HCl 1
Riboflavin 0.2
Thiamine·HCl 1
Vitamin B12 0.005