IgG is the most abundant antibody in FBS. The average level of IgG in normal fetal calf serum is approximately 70-300 µg/mL. RMBIO uses premium grade FBS as the starting material for our IgG Depleted FBS. We then utilize a series of chromatography procedures to reduce the IgG content. The resulting FBS has an average IgG level of less than 5 µg/mL and cell growth performance comparable to the starting material.

• Total IgG content below 5 µg/mL
• IgG depleted FBS performs well in monoclonal antibody production
• Triple 0.1 µm filtered
• Large lot sizes >1000 Liters available
• All fetal bovine serum is fully traceable and is sourced from audited suppliers
• Storage conditions: ≤ -10° C

Why use IgG depleted FBS?

IgG-depleted serum offers several benefits for various research and clinical applications. It reduces background interference in immunoassays, such as ELISAs, where high levels of IgG can cause non-specific binding and false positives. This serum is also valuable in cell culture and antibody production, minimizing the risk of cross-reactivity and enhancing the specificity of antibody-antigen interactions. Furthermore, it improves the accuracy and reliability of experimental results in immunological studies by eliminating the influence of endogenous IgG. Lastly, IgG-depleted serum is essential in the production of certain vaccines and therapeutic proteins, ensuring the purity and efficacy of the final product.

IgG Depleted Fetal Bovine Serum (FBS) specific product test requirements and typical values are given below: