DMEM-013: DMEM 1X, High Glucose, With HEPES, Without L-glutamine, Without Sodium Pyruvate
Dulbecco's Modified Eagle's Medium (DMEM), 1X, High Glucose (4500 mg/L), With HEPES (5,958 mg/L), Without L-glutamine, Without Sodium Pyruvate
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Catalog Number : DME-BBZ-XXX-013
DMEM 1X, High Glucose, With HEPES, Without L-glutamine, Without Sodium Pyruvate
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Product Details
Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional supplementary components, such as D-glucose and HEPES.
DME-BBZ-XXX-013 contains a high concentration of 4500 mg/L D-glucose and 25 mM of HEPES.
Storage/Stability
The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:
(1) Precipitate or particulate matter throughout the solution
(2) Cloudy appearance
(3) Color change
(4) pH change
The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Precautions
For research use only.
| Parameter | Method | Specification |
|---|---|---|
| Appearance | Visual | Red Transparent Solution |
| pH at RT After Addition of Sodium Bicrabonate | pH Meter | 7.0 - 7.6 |
| Osmolality | Chemistry Analyzer | 345 - 382 mOsm/kg H2O |
| Endotoxin | LAL | ≤ 1.0 EU/ml |
| Sterility | Sterilized by 0.2 um filtration system |
| CaCl2 (anhydrous) | 0.00 |
| Fe(NO3)3·9H2O | 0.10 |
| KCI | 400.00 |
| MgSO4 (anhydrous) | 0.0 |
| NaCI | 6400.00 |
| NaHCO3 | 3700.00 |
| NaH2PO4·H2O | 125.00 |
| D-glucose | 4500 |
| Phenol Red | 15.90 |
| HEPES | 5,958 |
| Sodium Pyruvate | 0.00 |
| L-Arginine·HCl | 84.00 |
| L-Cystine·2HCl | 62.57 |
| L-Glutamine | 0.00 |
| Glycine | 30.00 |
| L-Histidine·HCl·H2O | 42.00 |
| L-Isoleucine | 104.80 |
| L-Leucine | 104.80 |
| L-Lysine·HCl | 146.20 |
| L-Methionine | 30.00 |
| L-Phenylalanine | 66.00 |
| L-Serine | 42.00 |
| L-Threonine | 95.20 |
| L-Tryptophan | 16.00 |
| L-Tyrosine·2Na·2H2O | 103.79 |
| L-Valine | 93.60 |
| Calcium D-pantothenate | 0.00 |
| Choline Chloride | 4.00 |
| Folic Acid | 4.00 |
| Myo-inositol | 7.00 |
| Niacinamide | 4.00 |
| Riboflavin | 0.40 |
| Thiamine-HCI | 4.00 |
| Pyridoxine-HCl | 4.00 |