
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X) High Glucose
Widely used for mammalian cell culture, Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional supplementary components, such as D-glucose.
DME-BBZ-XXX-002 contains a high D-glucose concentration of 4500 mg/L, 584 mg/L L-glutamine, 4.0mg/L pyridoxine hydrochloride, and 110 mg/L sodium pyruvate. Important considerations when selecting a nutrient medium are: cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.
Parameter | Method | Specification |
---|---|---|
Appearance | Visual | Red Transparent Solution |
pH at RT Before Additions | pH Meter | 5.9 - 6.4 |
Osmolality | Chemistry Analyzer | 320 - 355 mOsm/kg H2O |
Endotoxin | LAL | ≤ 10 EU/ml |
Sterility | Sterilized by 0.2 um filtration system |
Glycine | 30 |
L-Arginine Hydrochloride | 84 |
L-Cystine 2HCl | 63 |
L-Glutamine | 584 |
L-Histidine Hydrochloride-H2O | 42 |
L-Isoleucine | 105 |
L-Leucine | 105 |
L-Lysine Hydrochloride | 146 |
L-Methionine | 30 |
L-Phenylalanine | 66 |
L-Serine | 42 |
L-Threonine | 95 |
L-Tryptophan | 16 |
L-Tyrosine Disodium Salt Dihydrate | 104 |
L-Valine | 94 |
Choline Chloride | 4 |
D-Calcium Pantothenate | 4 |
Folic Acid | 4 |
Niacinamide | 4 |
Pyridoxine Hydrochloride | 4 |
Riboflavin | 0.4 |
Thiamine Hydrochloride | 4 |
i-Inositol | 7.2 |
Calcium Chloride (CaCl2) (anhyd.) | 200 |
Ferric Nitrate (Fe(NO3)3”9H2O) | 0.1 |
Magnesium Sulfate (MgSO4) (anhyd.) | 97.67 |
Potassium Chloride (KCl) | 400 |
Sodium Chloride (NaCl) | 6400 |
Sodium Phosphate Monobasic (NaH2PO4-H2O) | 125 |
D-Glucose (Dextrose) | 4500 |
Phenol Red | 15 |
Sodium Pyruvate | 110 |