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DMEM/F-12 Ham 1:1 Mixture 1X, with L-glutamine, with HEPES in a 500 milliliter bottle.
DMEM/F-12 Ham 1:1 Mixture 1X, with L-glutamine, with HEPES in a 1000 milliliter bottle.

DMEM-003: DMEM/F-12 Ham 1:1 Mixture 1X, With L-glutamine, With HEPES

DMEM F-12 1:1 Nutrient Mixture Ham, 1X, with 365 mg/L L-glutamine and 15mM HEPES.
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Catalog Number : DME-BBZ-XXX-003
Dulbecco’s Modified Eagle’s Medium (DMEM) F-12 1:1 Nutrient Mixture
Case of 6 500ml bottles
Special Order for bulk

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Product Details

DMEM F-12 is a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture (Ham's F-12). Widely used as a basal medium, DMEM F-12 combines DMEM’s high amino acid, glucose, and vitamin concentrations with growth factors and hormones found in Ham’s F-12. The result is an ideal medium capable of culturing a variety of cell lines.

DME-BBZ-XXX-003 contains 365 mg/L L-glutamine and 15mM HEPES to compensate for the loss of buffering capacity incurred by eliminating serum. Important considerations when selecting a nutrient medium are: cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.


The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution

(2) Cloudy appearance

(3) Color change

(4) pH change

The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.


For research use only.

Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 6.9 - 7.5
Osmolality Chemistry Analyzer 280 - 326 mOsm/kg H2O
Endotoxin LAL ≤ 1 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 116.61
CuSO4·5H2O 0.0013
Fe(NO3)3·9H2O 0.05
FeSO4·7H2O 0.417
KCl 311.80
MgCl2 28.61
MgSO4 (anhydrous) 48.84
NaCl 6999.50
NaHCO3 1200.00
NaH2PO4·H2O 62.50
Na2HPO4 (anhydrous) 71.02
ZnSO4·7H2O 0.43
D-Glucose 3151.00
HEPES 3575.00
Na·Hypoxanthine 2.70
Linoleic Acid 0.040
DL-α-Lipoic Acid 0.105
Phenol Red-Na 8.60
Sodium Putrescine·2HCl 0.080
Sodium Pyruvate 55.00
Thymidine 0.37
L-Alanine 4.45
L-Arginine·HCl 147.50
L-Asparagine·H2O 7.501
L-Aspartic Acid 6.65
L-Cysteine·HCl·H2O 17.56
L-Cystine·2HCl 31.29
Glycine 18.75
L-Glutamic Acid 7.35
L-Glutamine 365.00
L-Histidine·HCl·H2O 31.48
L-Isoleucine 54.47
L-Leucine 59.05
L-Lysine·HCl 91.26
L-Methionine 17.24
L-Phenylalanine 35.48
L-Proline 17.25
L-Serine 26.25
L-Threonine 53.45
L-Tryptophan 9.02
L-Tyrosine·2Na·2H2O 55.79
L-Valine 52.85
Biotin 0.0036
Calcium D-pantothenate 2.24

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