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DMEM-008: DMEM 1X, High Glucose, With L-glutamine, With HEPES, Without Sodium Pyruvate

DMEM-008: DMEM 1X, High Glucose, With L-glutamine, With HEPES, Without Sodium Pyruvate

Dulbecco’s Modified Eagle’s Medium (DMEM) 1X, with 4500 mg/L D-glucose, 584 mg/L L-glutamine, 25 mM HEPES, no sodium pyruvate.
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Catalog Number : DME-BBZ-XXX-008
DMEM 1X, High Glucose, With L-glutamine, With HEPES, Without Sodium Pyruvate
Case of 6, 500mL bottles
Special Order for bulk

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Product Details

Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional supplementary components, such as D-glucose.

DME-BBZ-XXX-008 contains a high concentration of 4500 mg/L D-glucose, 584 mg/L L-glutamine, and 25 mM HEPES. Important considerations when selecting a nutrient medium include cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.


The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution

(2) Cloudy appearance

(3) Color change

(4) pH change

The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.


For In Vitro Use Only

Parameter Method Specification
Appearance Visual Red Transparent Solution
pH at RT Before Additions pH Meter 7.0 - 7.6
Osmolality Chemistry Analyzer 305 - 340 mOsm/kg H2O
Endotoxin LAL ≤ 1 EU/ml
Sterility Sterilized by 0.2 um filtration system

Components mg/L
CaCl2 (anhydrous) 200.00
Fe(NO3)3·9H2O 0.10
KCI 400.00
MgSO4 (anhydrous) 97.67
NaCI 6400.00
NaHCO3 2200.00
NaH2PO4·H2O 125.00
D-glucose 4500.00
Phenol Red 15.90
HEPES 5950.00
Sodium Pyruvate 0.00
L-Arginine·HCl 84.00
L-Cystine·2HCl 62.57
L-Glutamine 584.00
Glycine 30.00
L-Histidine·HCl·H2O 42.00
L-Isoleucine 104.80
L-Leucine 104.80
L-Lysine·HCl 146.20
L-Methionine 30.00
L-Phenylalanine 66.00
L-Serine 42.00
L-Threonine 95.20
L-Tryptophan 16.00
L-Tyrosine·2Na·2H2O 103.79
L-Valine 93.60
Calcium D-pantothenate 4.00
Choline Chloride 4.00
Folic Acid 4.00
Myo-inositol 7.00
Niacinamide 4.00
Riboflavin 0.40
Thiamine-HCI 4.00
Pyridoxine-HCl 4.00