DMEM-004: DMEM 1X, Low Glucose, With L-glutamine, With Sodium Pyruvate, Without HEPES
Dulbecco's Modified Eagle's Medium (DMEM), 1X, Low Glucose (1000 mg/L), With 110 mg/L sodium pyruvate, With 584 mg/L L-glutamine, Without HEPES
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Catalog Number : DME-BBZ-XXX-004
DMEM 1X, Low Glucose, With L-glutamine, With Sodium Pyruvate, Without HEPES
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Product Details
Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional components such as D-glucose
DME-BBZ-XXX-004 contains a low concentration of 1000 mg/L D-glucose, 584 mg/L L-glutamine, and 110 mg/L sodium pyruvate.
Storage/Stability
The liquid medium should be stored at 2~8°C away from direct sunlight. Deterioration of the liquid medium may be recognized by:
(1) Precipitate or particulate matter throughout the solution
(2) Cloudy appearance
(3) Color change
(4) pH change
The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Precautions
For research use only.
| Parameter | Method | Specification |
|---|---|---|
| Appearance | Visual | Red Transparent Solution |
| pH at RT Before Additions | pH Meter | 7.0 - 7.6 |
| Osmolality | Chemistry Analyzer | 305 - 340 mOsm/kg H2O |
| Endotoxin | LAL | ≤ 1 EU/ml |
| Sterility | Sterilized by 0.2 um filtration system |
| CaCl2 (anhydrous) | 200.00 |
| Fe(NO3)3·9H2O | 0.10 |
| Fe(NO3)3·9H2O | 0.05 |
| KCl | 400.00 |
| MgSO4 (anhydrous) | 97.67 |
| NaCl | 6400.00 |
| NaHCO3 | 3700.00 |
| NaH2PO4·H2O | 125.00 |
| D-Glucose | 1000.00 |
| Phenol Red-Na | 15.90 |
| HEPES | 0.00 |
| Sodium Pyruvate | 110.00 |
| L-Arginine·HCl | 84.00 |
| L-Cystine·2HCl | 62.57 |
| L-Glutamine | 584.00 |
| Glycine | 30.00 |
| L-Histidine·HCl·H2O | 42.00 |
| L-Isoleucine | 104.80 |
| L-Leucine | 104.80 |
| L-Lysine·HCl | 146.20 |
| L-Methionine | 30.00 |
| L-Phenylalanine | 66.00 |
| L-Serine | 42.00 |
| L-Threonine | 95.20 |
| L-Tryptophan | 16.00 |
| L-Tyrosine·2Na·2H2O | 103.79 |
| L-Valine | 93.60 |
| Calcium D-pantothenate | 4.00 |
| Choline Chloride | 4.00 |
| Folic Acid | 4.00 |
| Myo-inositol | 7.00 |
| Niacinamide | 4.00 |
| Riboflavin | 0.40 |
| Thiamine·HCl | 4.00 |
| Pyridoxine·HCl | 4.00 |