HMF-001: F-12 Nutrient Mixture (HAM’S F-12) 1X, With L-alanyl-L-glutamine, Without HEPES
HAM’s F-12 was originally developed in the 1960’s for serum-free growth of Chinese hamster ovary (CHO) cells. Since then, it has been widely used, with and without supplemental serum, to culture various mammalian and hybridoma cells.
HMF-BBZ-XXX-001 contains 217.23 mg/L L-alanyl-L-glutamine and is HEPES free. Important considerations when selecting a nutrient medium include: cell type, culture type (i.e., monolayer, suspension, or clonal) and the degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium type, required supplementation and physiological parameters required for a specific cell line.
Storage/Stability
The liquid medium should be stored at 2-8°C away from direct sunlight. Deterioration of the liquid medium may be recognized by:
- Precipitate or particulate matter visible throughout the solution
- Cloudy appearance
- Color change
- pH change
The nature of supplements added may affect the storage conditions and shelf life of the medium. Product label bears expiration date.
Precautions
For research use only.
| Parameter | Method | Specification |
|---|---|---|
| Appearance | Visual | Red Transparent Solution |
| pH at RT Before Additions | pH Meter | 7.2 - 7.8 |
| Osmolality | Chemistry Analyzer | 280 - 310 mOsm/kg H2O |
| Endotoxin | LAL | ≤ 1 EU/ml |
| Sterility | Sterilized by 0.2 um filtration system |
| CaCl2 (anhydrous) | 33.22 |
| KH2PO4 | 0.00 |
| CuSO4·5H2O | 0.0025 |
| FeSO4·7H2O | 0.83 |
| KCl | 223.65 |
| MgCl2 (anhydrous) | 57.22 |
| MgSO4 | 0.00 |
| NaCl | 7599.00 |
| NaHCO3 | 1176.00 |
| Na2HPO4 (anhydrous) | 142.04 |
| ZnSO4·7H2O | 0.86 |
| D-Glucose | 1801.60 |
| HEPES | 0.00 |
| Hypoxanthine·Na | 5.40 |
| Linoleic Acid | 0.09 |
| DL-α-Lipoic Acid | 0.21 |
| Phenol Red | 1.30 |
| Putrescine·2HCl | 0.16 |
| Sodium Pyruvate | 110.00 |
| Thymidine | 0.73 |
| L-Alanine | 8.91 |
| L-Alanyl-L-Glutamine | 217.23 |
| L-Arginine·HCl | 210.07 |
| L-Asparagine·H2O | 15.01 |
| L-Aspartic Acid | 13.30 |
| L-Cysteine·HCl·H2O | 35.12 |
| L-Glutamic Acid | 14.70 |
| L-Glutamine | 0.00 |
| Glycine | 7.51 |
| L-Histidine·3HCl·H2O | 20.96 |
| L-Isoleucine | 3.94 |
| L-Leucine | 13.12 |
| L-Lysine·HCl | 36.54 |
| L-Methionine | 4.48 |
| L-Phenylalanine | 4.96 |
| L-Proline | 34.53 |
| L-Serine | 10.51 |
| L-Threonine | 11.91 |
| L-Tryptophan | 2.04 |
| L-Tyrosine·2Na·2H2O | 7.78 |
| L-Valine | 11.71 |
| Biotin | 0.00733 |
| Calcium D-pantothenate | 0.24 |
| Choline Chloride | 13.96 |
| Folic Acid | 1.30 |
| Myo-Inositol | 18.02 |
| Niacinamide | 0.04 |
| Pyridoxine·HCI | 0.06 |
| Riboflavin | 0.04 |
| Thiamine·HCI | 0.34 |
| Vitamin B12 | 1.357 |