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Hanks' Balanced Salt Solution in a transparent 1000 milliliter bottle.
Hanks' Balanced Salt Solution in a transparent 500 milliliter bottle.

Hanks’ Balanced Salt Solution (HBSS) (1X) liquid

HBSS 1X, with calcium chloride, with magnesium chloride, without phenol red.
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Catalog Number : HBS-BBU
Hanks’ Balanced Salt Solutions (HBSS), Liquid
Case of 6 500mL bottles
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Product Details

Buffered saline solutions are balanced salt solutions formulated to maintain an osmotic balance similar to the natural condition of mammalian systems. Hanks’ balanced salt solution (HBSS) is widely used because its buffering capacity is effective at in vivo neutral pH. Other principal functions include: (1) dissolving or diluting materials for use in cell culture, (2) providing inorganic salts essential for basic cell metabolism, (3) maintaining osmotic balance, (4) buffering pH, and (5) eliminating certain components such as serum or trypsin.


HBSS should be stored at 2-30°C. after opening, store at 2-8°C. Deterioration of HBSS may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of HBSS. Product label bears expiration date.


For research use only.

Parameter Method Specification
CaCl2 140.00 mg/L
MgSO4 98.00 mg/L
KCl 400.00 mg/L
KH2PO4 60.00 mg/L
NaHCO3 350.00 mg/L
NaCl 8000.00 mg/L
Na2HPO4 48.00 mg/L
D-Glucose 1000.00 mg/L
pH at RT 7.0~7.6 mg/L
Osmolality 259~301 mOsm/kg H2O
Endotoxin LAL ≤ 1.0 EU/ml
Sterility USP <71> Pass

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