Newborn Bovine Calf Serum - Heat Inactivated
NBS can serve as a low-cost alternative to FBS while providing many of the same nutrients. Our NBS is heat inactivated at 56°C for 30 minutes.
NBS is manufactured and processed in our cGMP-compliant and FDA-registered facility according to the highest standards and is carefully monitored to ensure product safety and performance. These standards are built in to RMBIO's entire supply chain.
- Heat inactivated
- Average endotoxin level*: < 2 EU/ml (specification = ≤ 35 EU/ml)
- Average hemoglobin level*: < 20 mg/dl (specification = < 30 mg/dL)
- 9CFR tested
- 0.2 µm filtered
- US origin
- Manufactured in our cGMP-compliant and FDA-registered facility
- Large lot sizes of up to 5000L available
- Standard newborn calf serum packaged in 500mL or 1 L bottles. Custom packages are also available.
- Storage conditions: ≤ -10° C
*Data based on last 10 lots produced.
Heat Inactivation 101
Heat inactivation is an industry standard treatment that helps protect against growth inhibiting immune factors like serum complement that may be present in the serum. Serum complement can have undesirable impacts on immunological endpoints like detection of neutralizing antibodies, or on the proliferation of viruses. RMBIO manufactures heat inactivated bovine calf serum by raising the temperature of the serum to 56°C in a water bath and strictly maintaining the condition for 30 minutes.
Newborn Calf Serum specific product test requirements and typical values are given below:
|Hemoglobin||Spectro. Assay||< 30 mg/dL|
|Osmolality||By Osmometer||240 - 360 mOsm/Kg|
|pH||pH Meter||6.5 - 8.5|
|Total Protein||Chemistry Analyzer||3.0 - 6.0 g/gL|
|Endotoxin||LAL||≤ 35 EU/ml|
|Mycoplasma||9CFR 113.28||Not Detected|
|Sterility||9CFR 113.26||No Growth|
|Viral Analysis||9CFR 113.53||Not Detected
Product is heated at 56°C for 30 minutes to inactivate serum complement.